hfq [2019-02-19 19:04:29]

RNA chaperone

locus: BSU17340

pI: 8.70

mw: 8.38 kDa

protein length: 73 aa

gene length: 222 bp

function: unknown

product: RNA chaperone

essential: no

synonyms: ymaH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]

Gene

Coordinates: 1,867,373 1,867,594

The protein

Protein family

• hfq family (according to Swiss-Prot)

Structure

• [PDB|3HSB] (complex with an RNA aptamer) [Pubmed|22053080]

Expression and Regulation

Operons

• genes: [gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
  description: [pubmed|22383849]

• genes: [gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
  description: [pubmed|22383849]

  regulation

  • expressed during [SW|sporulation ][Pubmed|22383849]

• genes: [gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
  description: [Pubmed|23457461]

  regulation

  • expression (mRNA levels) is quite constant during growth in minimal medium [Pubmed|23457461]

Biological materials

Mutant

• GP22 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::cat), available in the [SW|Jrg Stlke]'s lab
• BKE17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT
• BKK17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT

Expression vectors

• pGP2166: expression of ''hfq''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jrg Stlke]'s lab

lacZ fusion

• pGP460 (in [protein|search|pAC7]), available in [SW|Jrg Stlke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

FLAG-tag construct

• GP1067 (spc, based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab

References

Reviews

• 17395525,15009882,21760622,26773797

Original publications

• 12850135,20445260,23457461,22965117,22053080,24932523,25150227,25915524,27049793,27581927,12093755