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hfq [2019-02-19 19:04:29]
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hfq [2019-02-19 19:04:29]

RNA chaperone
locus
BSU17340
pI
8.70
mw
8.38 kDa
protein length
gene length
222 bp Sequence Blast
function
unknown
product
RNA chaperone
essential
no
synonyms
ymaH

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
  • Gene

    Coordinates
    1,867,373 1,867,594

    The protein

    Protein family

  • hfq family (according to Swiss-Prot)
  • Structure

  • [PDB|3HSB] (complex with an RNA aptamer) [Pubmed|22053080]
  • Expression and Regulation

    Operons

    genes
    [gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
    description
    [pubmed|22383849]

    regulation

  • expressed during [SW|sporulation ][Pubmed|22383849]
  • view in new tab

    genes
    [gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
    description
    [Pubmed|23457461]

    regulation

  • expression (mRNA levels) is quite constant during growth in minimal medium [Pubmed|23457461]
  • view in new tab

    Biological materials

    Mutant

  • GP22 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::cat), available in the [SW|Jrg Stlke]'s lab
  • BKE17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT
  • BKK17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT
  • Expression vectors

  • pGP2166: expression of ''hfq''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jrg Stlke]'s lab
  • lacZ fusion

  • pGP460 (in [protein|search|pAC7]), available in [SW|Jrg Stlke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab
  • FLAG-tag construct

  • GP1067 (spc, based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab
  • References

    Reviews

  • 17395525,15009882,21760622,26773797
  • Original publications

  • 12850135,20445260,23457461,22965117,22053080,24932523,25150227,25915524,27049793,27581927,12093755